Arousal effects on Fitness-to-Drive assessment: algorithms and experiments

Several elements can affect the drivers' behaviour while they are performing driving activities. Ranging from visual to cognitive distractions, emotions and other drivers' conditions (that could emerge from biometric data, such as temperature, heartbeat, pressure, etc.) can play a significant role, performing as a factor that can increase drivers' response time. This could be crucial in avoiding dangerous situations and in deciding and performing actions that could influence the happening of car accidents. This paper introduces the concept of the "Fitness-to-Drive" index and aims to evaluate how the arousal effects can influence the drivers' status. The paper presents some experimental evaluations we have conducted on a driver simulator, discussing the obtained results

Protective effect of chlorpromazine on endotoxin toxicity and TNF production in glucocorticoid-sensitive and glucocorticoid-resistant models of endotoxic shock

The present study was designed to define the potential ofchlorpromazine (CPZ) as a protective agent against lipopolysaccharide (LPS) toxicity in comparison with glucocorticoids, and to obtain initial correlations with its effects on the levels of tumor necrosis factor (TNF), a pivotal mediator of endotoxic shock. It was found that CPZ protects mice, normal or adrenalectomized, and guinea pigs against lethality of LPS, and inhibited TNF serum levels, like dexamethasone (DEX), a well-known inhibitor ofTNF synthesis. CPZ protected against LPS lethality when administered 30 minutes (min) before, simultaneously, or up to 10 min after LPS and was ineffective when given 30 min after LPS, paralleling the inhibitory effect on TNF production. In another experimental model, where mice were sensitized to LPS toxicity by actinomycin D, CPZ significantly inhibited LPS lethality and hepatotoxicity, whereas under these conditions DEX was inactive. These experiments indicate that CPZ has a protective action in both glucocorticoid-sensitive and -resistant models of endotoxic shock

Beam-Dependent Active Array Linearization by Global Feature-Based Machine Learning

An approach based on machine learning is proposed for the global linearization of microwave active beamforming arrays. The method allows for the low-complexity real-time update of the digital predistortion (DPD) coefficients by exploiting order-reduced model features, hence avoiding the need for repeated local DPD identification steps across the various operating conditions of the beamformer (e.g., different beam angles or RF power levels). The validation is performed by over-the-air (OTA) measurements of a 1×4 array operating at 28 GHz across 100-MHz modulation bandwidth (BW)

Automatic Optimization of Input Split and Bias Voltage in Digitally Controlled Dual-Input Doherty RF PAs

Digitally controlled Dual-Input Doherty Power Amplifiers (DIDPAs) are becoming increasingly popular due to the flexible input signal splitting between the main and auxiliary stages. Nevertheless, the presence of many degrees of freedom, e.g., input amplitude split and phase displacement as well as biasing for multiple stages, often involves inefficient trial-and-error procedures to reach a suitable PA performance. This article presents automated parameter setting based on coordinate descent or Bayesian optimizations, demonstrating an improvement in the performance in terms of RF output power and power-added efficiency (PAE) in the presence of broadband-modulated signals, yet maintaining suitable linear behavior for, e.g., communications applications

Low oxygen tension primes aortic endothelial cells to the reparative effect of tissue-protective cytokines

Erythropoietin (EPO) has both erythropoietic and tissue-protective properties. The EPO analogues carbamylated EPO (CEPO) and pyroglutamate helix B surface peptide (pHBSP) lack the erythropoietic activity of EPO but retain the tissue-protective properties that are mediated by a heterocomplex of EPO receptor (EPOR) and the β common receptor (βCR). We studied the action of EPO and its analogues in a model of wound healing where a bovine aortic endothelial cells (BAECs) monolayer was scratched and the scratch closure was assessed over 24 h under different oxygen concentrations. We related the effects of EPO and its analogues on repair to their effect on BAECs proliferation and migration (evaluated using a micro-Boyden chamber). EPO, CEPO and pHBSP enhanced scratch closure only at lower oxygen (5%), while their effect at atmospheric oxygen (21%) was not significant. The mRNA expression of EPOR was doubled in 5% compared to 21% oxygen, and this was associated with increased EPOR assessed by immunofluorescence and Western blot. By contrast βCR mRNA levels were similar in 5% and 21% oxygen. EPO and its analogues increased both BAECs proliferation and migration, suggesting that both may be involved in the reparative process. The priming effect of low oxygen tension on the action of tissue-protective cytokines may be of relevance to vascular disease, including atherogenesis and restenosis

Erythropoietin (EPO) increases myelin gene expression in CG4 oligodendrocyte cells through the classical EPO receptor

Erythropoietin (EPO) has protective effects in neurodegenerative and neuroinflammatory diseases, including in animal models of multiple sclerosis, where EPO decreases disease severity. EPO also promotes neurogenesis and is protective in models of toxic demyelination. In this study, we asked whether EPO could promote neurorepair by also inducing remyelination. In addition, we investigated whether the effect of EPO could be mediated by the classical erythropoietic EPO receptor (EPOR), since it is still questioned if EPOR is functional in non-hematopoietic cells. Using CG4 cells, a line of rat oligodendrocyte precursor cells, we found that EPO increases the expression of myelin genes (myelin oligodendrocyte glycoprotein (MOG) and myelin basic protein (MBP)). EPO had no effect in wild-type CG4 cells, which do not express EPOR, whereas it increased MOG and MBP expression in cells engineered to overexpress EPOR (CG4-EPOR). This was reflected in a marked increase in MOG protein levels, as detected by western blot. In these cells, EPO induced by 10-fold the early growth response gene 2 (Egr2), which is required for peripheral myelination. However, Egr2 silencing with a siRNA did not reverse the effect of EPO, indicating that EPO acts through other pathways. In conclusion, EPO induces the expression of myelin genes in oligodendrocytes and this effect requires the presence of EPOR. This study demonstrates that EPOR can mediate neuroreparative effects